Immuno - pcr for il - 6 was established by use of 1ng / l of the biotinylated dna as template . this immuno - pcr has a detection limit of 1fg / ml of pil - 6 that is 105 times lower than that of indirect elisa , and is the most sensitive method to detect il - 6 up to date 通过与elisa法比较，发现新建立的免疫pcr法灵敏度为1fg ml猪il - 6 ，是elisa法的10 ~ 5倍，是目前已报道的检测猪il - 6最灵敏的方法。
By immune the animal with the protein , the antibody which is anti - gst - cpti fusion protein was prepared . lt was testified that the antibody can special immunological recognition the protein gst - cpti , gstand cpti by indirect elisa . the coefficient of correlation is significant and the potency is more than 1 : 8000 对其进行细菌表达后，利用glutathionesepharose4b亲和柱纯化获得gst - cpti融合蛋白，免疫动物后制备了相应的抗gst - cpti融合蛋白抗体。
After the protein refolding of denaturant inclusion body following dialysis , we got the pure recombinant gst - eo protein by gst affinity columns . using the purified protein as coating antigen , an indirect elisa were developed for detecting the anti - eo antibody in the csfv serum by exploring the concentration of coating an tigen and dilution degree of serum 使用分步透析法对变性的包涵体进行复性，将复性蛋白过gst亲和层析柱得到纯化的gst - eo融合蛋白。以gst - eo融合蛋白为诊断抗原，初步建立了用间接elisa检测猪瘟血清eo抗体的方法。
From dead chicken which infected infectious stunting syndrom of our province , one virue was isolated using spfeggs , chicken embryo fibroblast , mdck18 , and vero cell . this virus was unable to agglutinate chicken erythrocytes . in order to definite the pathogeny of infectious stunting syndrom . physical and chemical specific property , types of the nucleic acid of the isolated virus , recurrent infection and other biological property determination and indirect elisa test proved it as a parvoviruses like strain of chicken 为确定该病的病原，对所分离病毒进行了理化特性测定、病毒核酸型别测定、动物回归试验等生物学特性测定，证明该分离病毒与细小病毒科( parvovirdae )细小病毒属( parvovirus )的鸡细小病毒( chickenparvovirus )特性基本相符，核酸型为dna型。
After purification and absolv in 8mol / l urea . and then injected rabbits 3 times with 14 days interval . by analysis of indirect elisa , the anti - pea - h3 antibody appear in the rabbit serum induced by the recombinant fusion protein , the highest antibody level was observed during 7 - 14days after the final inoculation 提取重组质粒并转化大肠杆菌bl21 ( de3 ) ，经iptg诱导阳性菌，对菌体总蛋白进行sds - page电泳分析，结果表明bl21 ( de3 )能有效表达目的蛋白，薄层扫描计算分析，显示表达蛋白带密度积分占总蛋白量的16 . 28 。
Methods : the balb / c mouse is immunized with gene recombinant antigen p24 for four times in 2 months . the spleen cells of immunized mouse is hybridized with sp2 / 0 by peg , and the positive cell clones secreting the antibody to antigen p24 are detected by indirect elisa . through three clonings less diversed anti - p24 hybridoma cells are gained 方法：基因工程p24抗原免疫小鼠4次，历时2个月，取脾细胞与骨髓瘤细胞株sp2 0 ，用peg融合， hat选择培养和间接elisa筛选分泌抗p24抗体阳性的杂交瘤细胞，三次克隆化后得稳定分泌抗p24抗体的杂交瘤细胞株。
Colibacollosis serotype k . 99 positive sera in ducks and duck plague positive sera were tested , whereas , the results of detection to r . a . sera were positive . the sensitivity of indirect elisa were 50 to 100 times ( serotype 1 ) , ( 2 ) 25 to 100 times ( serotype 2 ) , ( 3 ) 12 to 100 times ( serotype 4 ) and 25 to 200 times ( serotype 5 ) th an micro - agglutination test 包被鸭疫里默氏杆菌抗原时，对鸭抗5 ： a多杀性巴氏杆菌阳性血清、鸭抗型鸭病毒性肝炎阳性血清、鸭抗鸭源大肠杆菌k _ ( 88 )阳性血清、鸭抗鸭源肠炎沙门氏菌阳性血清、鸭抗鸭源大肠杆菌k _ ( 99 )阳性血清、鸭抗鸭瘟阳性血清检测结果呈阴性。
2 - e4 - a and 82 - 6 are hybridized during their log growing time , and the hybrid - hybridomas are cloned for 3 times and produce 6 hybrid - hybridoma cells . the chromatosome of hybrid - hybridoma 3 - hu and hybridoma 2 - e4 - a and s2 - b are counted , and the antibody of ascites fluid or culture supernatant of 3 - hn is prepared . the positive clones are detected by three methods at the same time : rbc agglutination for monospecific anti - human rbc type a antibody , indirect elisa for anti - p24 antibody , and rbc solid - phase adherence for bispecific antibody 选其中一株3 - h _ ( 11 )做杂交-杂交瘤细胞染色体计数，同时计数两母株2 - e _ 4 - a和s _ 2 - b的染色体数：制备腹水型和上清型抗体，用三种方法同时检测其中的双特异性抗体、单特异性抗人红细胞抗体和抗p24抗体，即红细胞固相吸附法测双特异中文摘要性抗体，红细胞凝集试验测单特异性抗人a型红细胞抗体，间接elisa法测抗p24抗体；用腹水型抗体做耐热性及耐冻融实验。
3 immunogenicity of m3m4 loop recombinant peptide antigen of nr1 , neuroexcitotoxicity protective effects and their mechanisms study of antiserum against m3m4 ( 1 ) to explore the immunogenicity of m3m4 loop recombinant peptide vaccine , m3m4 recombinant peptide was injected subcutaneously to immunize balb / c mice ( h - 2 ) , cd4 / cd8 t lymphocytes subsets , the cytokine levels in serum and the liter of specific humoral response were detected with facs , indirect elisa and elisa separately ，抗血清还可以结合合成肤nps ，证明该表位具有免疫原性和抗原性。收集抗血清用于功能检测。 (二) m3m4片段抗血清抗兴奋毒性损害作用以台盼蓝染色法和原位末端标记( tun ’ el )法观察抗血清对原代培养海马神经元兴奋毒性坏死和凋亡的保护效应。
107 + 2 . 819x ( serotype l ) , log [ et reciprocal ] = 1 . 019 + 2 . 935x ( serotype 2 ) , ( 3 ) log [ et reciprocal ] = 0 . 99 + 2 . 709x ( serotype 4 ) and log [ et reciprocal ] = 1 . 052 + 2 . 953x ( serotype 5 ) . the derived regression lines ( equations ) were used to transfer the optical density value ( od ) obtained from a single 1 : 100 dilution of any unknown serum sample of duck into elisa titer . the indirect elisa technique were used to monitor the variety of the mean antibodies titers of vaccinated ducks with the oil - emusified formalin - inactived r . a 间接elisa方法的灵敏度是微量凝集试验的50 - 100倍(血清1型鸭疫里默氏杆菌) ， 25 - 100倍(血清2型鸭疫里默氏杆菌) ， 12 - 100倍(血清4型鸭疫里默氏杆菌) ， 25 - 200倍(血清5型鸭疫里默氏杆菌) 。
